Highly Sensitive Detection of Bensulfuron-methyl by Time-Resolved Fluoroimmunoassay Using a Tetradentate .BETA.-Diketonate Europium Chelate as a Label.
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چکیده
منابع مشابه
Immunofluorometry of choriogonadotropin by time-resolved fluorescence spectroscopy, with a new europium chelate as label.
We describe a new "sandwich"-type non-isotopic immunoassay for human choriogonadotropin (hCG) in serum. In the assay, hCG is captured by a beta-subunit-specific monoclonal antibody, which is immobilized in a white microtiter well. The sandwich is completed by adding a second biotinylated monoclonal antibody specific for the whole hCG molecule. The degree of binding of biotinylated antibody, whi...
متن کاملA novel bifunctional europium chelate applied in quantitative determination of human immunoglobin G using time-resolved fluoroimmunoassay.
The authors demonstrate herein a novel time-resolved fluoroimmunoassay (TRFIA) protocol for quantification of human IgG with the new bifunctional chelate Eu(TTA)₃(5-NH₂-phen) (ETNP) labeling the goat anti-human IgG. The immunoassay was conducted by following the typical procedure for sandwich-type immunoreactions. Goat anti-human IgG was immobilized on aldehyde-modified glass slides. The human ...
متن کاملTime-Resolved Luminescence Microscopy and Microarray Using Europium Chelate Labels
The luminescent Eu complex, DTBTA-Eu, is highly stable in terms of ligand-metal dissociation, and can be labelled to amino groups of proteins and other bio-molecules. Owing to the long lifetime of Eu luminescence, time-resolved measurement of DTBTA-Eu removes background fluorescence and enhances the sensitivity of the labelled materials. This property was applied to time-resolved luminescence m...
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A two-site assay was developed by use of the "dissociation and enhancement lanthanide fluoroimmunoassay" (DELFIA) technique for determination of salmon calcitonin (SCT) in serum after administration to osteoporotic patients. Polyclonal antibodies were produced in rabbits immunized with SCT coupled to ovalbumin. After affinity purification, the antibodies were used both as immobilized capture an...
متن کاملPhotostability of a highly luminescent europium beta-diketonate complex in imidazolium ionic liquids.
A high quantum yield and an enhanced photostability was found for a europium(III) tetrakis(2-thenoyltrifluoroacetonate) complex after dissolving the complex in a weakly-coordinating imidazolium ionic liquid.
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 1999
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.15.125